Blocking Assay:Article Title: In vitro effects of polystyrene microplastics exposure on adipose tissue dysfunction.
Article Snippet: .. After blocking, the membranes were incubated overnight with specific primary antibodies: rabbit anti C/ EBP-β (LAP) polyclonal antibody (#3087 Cell Signaling Technology) (1:1000), mouse anti-PPARγ monoclonal antibody (#sc-7273 Santa Cruz Biotechnology) (1:1000), rabbit anti p-eIF2α (Ser51)(D9G8) monoclonal antibody (#3398 Cell Signaling Technology) (1:500), rabbit anti-XBP-1 s (E9V3E) monoclonal antibody (#40435 Cell Signaling Technology) (1:500), mouse anti-caspase − 3 monoclonal antibody (#sc7273 Santa Cruz Biotechnology) (1:500). .. This was followed by a 2-h incubation with peroxidase-conjugated secondary antibody HRP labeled goat antirabbit Ig (#7074 Cell Signaling Technology) (1:6000) or goat anti-Mouse IgM Secondary Antibody, HRP conjugate (#7076 Cell Signaling Technology) (1:6000), and visualized with an ECL plus detection system (Amersham; GE Healthcare Life Sciences).
Incubation:Article Title: In vitro effects of polystyrene microplastics exposure on adipose tissue dysfunction.
Article Snippet: .. After blocking, the membranes were incubated overnight with specific primary antibodies: rabbit anti C/ EBP-β (LAP) polyclonal antibody (#3087 Cell Signaling Technology) (1:1000), mouse anti-PPARγ monoclonal antibody (#sc-7273 Santa Cruz Biotechnology) (1:1000), rabbit anti p-eIF2α (Ser51)(D9G8) monoclonal antibody (#3398 Cell Signaling Technology) (1:500), rabbit anti-XBP-1 s (E9V3E) monoclonal antibody (#40435 Cell Signaling Technology) (1:500), mouse anti-caspase − 3 monoclonal antibody (#sc7273 Santa Cruz Biotechnology) (1:500). .. This was followed by a 2-h incubation with peroxidase-conjugated secondary antibody HRP labeled goat antirabbit Ig (#7074 Cell Signaling Technology) (1:6000) or goat anti-Mouse IgM Secondary Antibody, HRP conjugate (#7076 Cell Signaling Technology) (1:6000), and visualized with an ECL plus detection system (Amersham; GE Healthcare Life Sciences).
Article Title: Low concentrations of antimony impair adipogenesis and endoplasmic reticulum homeostasis during 3T3-L1 cells differentiation.
Article Snippet: .. The membranes were blocked, for 1 h at room temperature, with Tris-buffered saline containing 5% of lyophilized non-fat milk and then incubated overnight with specific primary antibodies: rabbit anti C/EBP-β (LAP) polyclonal antibody (#3087 Cell Signaling Technology) (1:1000), mouse anti-PPAR-γ monoclonal antibody (#sc-7273 Santa Cruz Biotechnology) (1:1500), rabbit anti–NF–κB p65 polyclonal antibody (#PA5-16545 Invitrogen) (1:1000), rabbit anti-Phospho-IKK α/β (Ser176/180) monoclonal antibody (#sc-2697 Cell Signaling Technology) (1:1000), Rabbit anti-IKKβ monoclonal antibody (#8943 Cell Signaling Technology) (1:1000), mouse anti-Cox-2 monoclonal antibody (#sc-160107 Santa Cruz Biotechnology) (1:200), rabbit anti-XBP1s (E9V3E) monoclonal antibody (#40435 Cell Signaling Technology) (1:500), rabbit anti-Phospho-eIF2α (Ser51)(D9G8) monoclonal antibody (#3398 Cell Signaling Technology) (1:500), rabbit anti-eIF2α (D7D3) monoclonal antibody (#5324 Cell Signaling Technology) (1:1000), rabbit anti p21 Waf1/Cip1 monoclonal antibody (#sc-271610 Santa Cruz Biotechnology, Dallas, TX, USA) (1:500), rabbit anti-β-Actin monoclonal antibody (#4970 Cell Signaling Technology) (1:6000), rabbit anti-Lamin-B monoclonal antibody (#13435 Cell Signaling Technology) (1:1500), followed by 2 h incubation with peroxidaseconjugated secondary antibody HRP labeled goat antirabbit Ig (#7074 Cell Signaling Technology) (1:6000), goat anti-Mouse IgM Secondary Antibody, HRP conjugate (#7076 Cell Signaling Technology) (1:6000), and visualized with an ECL plus detection system (Amersham; GE Healthcare Life Sciences). .. Blots were detected using High Performance chemiluminescence film (Amersham HyperfilmTM ECL; GE Healthcare Life Sciences.
Saline:Article Title: Low concentrations of antimony impair adipogenesis and endoplasmic reticulum homeostasis during 3T3-L1 cells differentiation.
Article Snippet: .. The membranes were blocked, for 1 h at room temperature, with Tris-buffered saline containing 5% of lyophilized non-fat milk and then incubated overnight with specific primary antibodies: rabbit anti C/EBP-β (LAP) polyclonal antibody (#3087 Cell Signaling Technology) (1:1000), mouse anti-PPAR-γ monoclonal antibody (#sc-7273 Santa Cruz Biotechnology) (1:1500), rabbit anti–NF–κB p65 polyclonal antibody (#PA5-16545 Invitrogen) (1:1000), rabbit anti-Phospho-IKK α/β (Ser176/180) monoclonal antibody (#sc-2697 Cell Signaling Technology) (1:1000), Rabbit anti-IKKβ monoclonal antibody (#8943 Cell Signaling Technology) (1:1000), mouse anti-Cox-2 monoclonal antibody (#sc-160107 Santa Cruz Biotechnology) (1:200), rabbit anti-XBP1s (E9V3E) monoclonal antibody (#40435 Cell Signaling Technology) (1:500), rabbit anti-Phospho-eIF2α (Ser51)(D9G8) monoclonal antibody (#3398 Cell Signaling Technology) (1:500), rabbit anti-eIF2α (D7D3) monoclonal antibody (#5324 Cell Signaling Technology) (1:1000), rabbit anti p21 Waf1/Cip1 monoclonal antibody (#sc-271610 Santa Cruz Biotechnology, Dallas, TX, USA) (1:500), rabbit anti-β-Actin monoclonal antibody (#4970 Cell Signaling Technology) (1:6000), rabbit anti-Lamin-B monoclonal antibody (#13435 Cell Signaling Technology) (1:1500), followed by 2 h incubation with peroxidaseconjugated secondary antibody HRP labeled goat antirabbit Ig (#7074 Cell Signaling Technology) (1:6000), goat anti-Mouse IgM Secondary Antibody, HRP conjugate (#7076 Cell Signaling Technology) (1:6000), and visualized with an ECL plus detection system (Amersham; GE Healthcare Life Sciences). .. Blots were detected using High Performance chemiluminescence film (Amersham HyperfilmTM ECL; GE Healthcare Life Sciences.
Labeling:Article Title: Low concentrations of antimony impair adipogenesis and endoplasmic reticulum homeostasis during 3T3-L1 cells differentiation.
Article Snippet: .. The membranes were blocked, for 1 h at room temperature, with Tris-buffered saline containing 5% of lyophilized non-fat milk and then incubated overnight with specific primary antibodies: rabbit anti C/EBP-β (LAP) polyclonal antibody (#3087 Cell Signaling Technology) (1:1000), mouse anti-PPAR-γ monoclonal antibody (#sc-7273 Santa Cruz Biotechnology) (1:1500), rabbit anti–NF–κB p65 polyclonal antibody (#PA5-16545 Invitrogen) (1:1000), rabbit anti-Phospho-IKK α/β (Ser176/180) monoclonal antibody (#sc-2697 Cell Signaling Technology) (1:1000), Rabbit anti-IKKβ monoclonal antibody (#8943 Cell Signaling Technology) (1:1000), mouse anti-Cox-2 monoclonal antibody (#sc-160107 Santa Cruz Biotechnology) (1:200), rabbit anti-XBP1s (E9V3E) monoclonal antibody (#40435 Cell Signaling Technology) (1:500), rabbit anti-Phospho-eIF2α (Ser51)(D9G8) monoclonal antibody (#3398 Cell Signaling Technology) (1:500), rabbit anti-eIF2α (D7D3) monoclonal antibody (#5324 Cell Signaling Technology) (1:1000), rabbit anti p21 Waf1/Cip1 monoclonal antibody (#sc-271610 Santa Cruz Biotechnology, Dallas, TX, USA) (1:500), rabbit anti-β-Actin monoclonal antibody (#4970 Cell Signaling Technology) (1:6000), rabbit anti-Lamin-B monoclonal antibody (#13435 Cell Signaling Technology) (1:1500), followed by 2 h incubation with peroxidaseconjugated secondary antibody HRP labeled goat antirabbit Ig (#7074 Cell Signaling Technology) (1:6000), goat anti-Mouse IgM Secondary Antibody, HRP conjugate (#7076 Cell Signaling Technology) (1:6000), and visualized with an ECL plus detection system (Amersham; GE Healthcare Life Sciences). .. Blots were detected using High Performance chemiluminescence film (Amersham HyperfilmTM ECL; GE Healthcare Life Sciences.
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